Journal: Npj Dementia

Article Title: Targeted profiling of the serum proteome associates increased FGF-23 levels with postoperative delirium in cardiac surgical patients

doi: 10.1038/s44400-026-00084-w

Figure Lengend Snippet: a Volcano plot representing the log 2 -fold change at postoperative day 1 comparing the delirium and no delirium groups. Dotted lines indicate significance cutoffs of p < 0.05 and fold change < 2/3-fold or > 3/2-fold. b Relative protein concentrations of the 2 proteins with differential regulation in the delirium group. c An independent validation study of the relative protein concentrations of the 2 proteins identified in the primary cohort. d A second independent validation study of FGF-23 concentrations using ELISAs. Mann-Whitney U * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Preoperative and postoperative serum levels of the following factors or proteins were determined by ELISA according to the manufacturers’ protocols: Vitamin D (Total 25-OH Vitamin D ELISA Kit, cat. # 80987; Crystal Chem, Elk Grove Village, IL, USA), FGF-23 (Human FGF-23 ELISA Kit, ELH-FGF23; RayBio, Norcross, GA, USA), Klotho (Human Klotho DuoSet ELISA, DY5334-05; R&D Systems, Minneapolis, MN, USA), and IL-6 (Human IL-6 ELISA Kit, KAC1261; Life Technologies, Frederick, MD, USA).

Techniques: Biomarker Discovery, MANN-WHITNEY

Journal: Npj Dementia

Article Title: Targeted profiling of the serum proteome associates increased FGF-23 levels with postoperative delirium in cardiac surgical patients

doi: 10.1038/s44400-026-00084-w

Figure Lengend Snippet: a Baseline comparison between IL-6 and FGF-23 levels. The solid line indicates the least squares regression, and the dotted lines show the 95% CI. b Correlation analysis of NfL and FGF-23 serum levels on postoperative day 1. c Correlation analysis between PEA and ELISA measurements of postoperative FGF-23 serum levels. d Receiver operative characteristic (ROC) curves and the area under the curve (AUC) values for FGF-23 levels at preoperative, postoperative day 1, and perioperative change, as discriminators of delirium.

Article Snippet: Preoperative and postoperative serum levels of the following factors or proteins were determined by ELISA according to the manufacturers’ protocols: Vitamin D (Total 25-OH Vitamin D ELISA Kit, cat. # 80987; Crystal Chem, Elk Grove Village, IL, USA), FGF-23 (Human FGF-23 ELISA Kit, ELH-FGF23; RayBio, Norcross, GA, USA), Klotho (Human Klotho DuoSet ELISA, DY5334-05; R&D Systems, Minneapolis, MN, USA), and IL-6 (Human IL-6 ELISA Kit, KAC1261; Life Technologies, Frederick, MD, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay